Investigation in biology is similar to estimation of biological molecules in cells and tissues. Usually to estimate hormones, proteins, DNA, etc., in cells optical properties such as behavior of matter upon interacting with light etc of molecules are broadly used. A variety of spectrophotometers have been procured to study optical properties of biological molecules. The newly created centralized facility of Spectroscopy at the BCSIR Laboratory Chittagong permits comprehensive analysis of molecules in cells. High throughput quantification equipment including ” UV/Vis and IR Spectroscopy , Atomic Emission Spectroscopy ” allows hundreds of measurements simultaneously. The uniqueness of the Spectroscopy facility at the BCSIR Laboratory Chittagong is to have instruments at one centralized point for all spectroscopic investigations of biomolecules.